Calcium-Binding Protein Protocols: Volume 2

Description

Calcium-Binding Protein Protocols: Volume 2

 Book Properties
ISBN: 0896036898
Title: Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology)
Author:
Publisher: Humana Press
Publication Date: 2001-12-15
Number Of Pages: 415
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Download Details:Methods in Molecular Biology, vol.173

Blurb for Volume 2 Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting–edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium–binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action. Single Blurb for Both Volumes Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. The first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second volume focuses on cutting–edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium–binding proteins in cells.

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1. Ebooks list page : 103
2. Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Histories by Hans J. Vogel
3. Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology) by Hans J. Vogel
4. Protein Blotting and Detection: Methods and Protocols (Methods in Molecular Biology Volume 536)
5. Viral Applications of Green Fluorescent Protein: Methods and Protocols (Methods in Molecular Biology Volume 515)
6. Calcium Signaling Protocols (Methods in Molecular Biology) by David G. Lambert
7. Mechanisms and Pathways of Heterotrimeric G Protein Signaling, Volume 74 (Advances in Protein Chemistry) by Stephen Sprang
8. Regulation of membrane protein transport by ubiquitin and ubiquitin-binding proteins
9. Calcium Signalling and Disease: Molcular pathology of calcium (Subcellular Biochemistry)
10. Calcium Signalling and Disease: Molcular pathology of calcium (repost)
11. Calcium Signalling and Disease: Molcular Pathology of Calcium (2007)
12. Calcium Signalling and Disease: Molcular pathology of calcium
13. Protein-DNA Interactions, Volume 208: Volume 208: Protein - Dna Interactions (Methods in Enzymology)
14. RNA-Protein Interaction Protocols
15. Adhesion Protein Protocols
16. Protein Phosphate Protocols