Cell Imaging Techniques: Methods and Protocols (Me...

Description

Title: Cell Imaging Techniques: Methods and Protocols (Methods in Molecular Biology)
Description:
Pages: 490

The editors (both of the U. of Vermont's Department of Pathology) present an

"eclectic" collection of what they consider state-of-the- art methods for imaging

cells and molecules. The 21 papers begin with light microscopic methods for

observing mRNA, calcium, and collagen. Next come contributions discussing confocal

scanning laser microscopy, quantitative computer-assisted image analysis, laser

scanning cytometry, laser capture microdissection, microarray image scanning,

near-field scanning optical microscopy, atomic force microscopy, and reflection

contrast microscopy. Three dimensional analysis of single particles by electron

microscopy is discussed in a pair of chapters dealing first with sample

preparation and data acquisition, and then with image processing. The volume

concludes with a look at new microbiopsy system that enables rapid preparation of

tissue for high-pressure freezing. The protocols follow the series format,

offering step-by-step laboratory instructions, discussion of the principles behind

the technique, lists of equipment and reagents, and advice on troubleshooting and

pitfalls.

Links

http://linkgrabber.blogspot.com/2006/11/cell-imaging-techniques-methods-and.html

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