DNA Microarrays, Part B:

Description

Title: DNA Microarrays, Part B:
Description:
Databases and Statistics, Volume 411 (Methods in Enzymology)
Pages: 512

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is

now possible to take many million measurements from a single experiment. These two volumes,

Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular

biologist through the process of planning, performing, and publishing microarray results.
Part A starts with an overview of a number of microarray platforms, both commercial and

academically produced and includes wet bench protocols for performing traditional expression

analysis and derivative techniques such as detection of transcription factor occupancy and

chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B.

These techniques are well rooted in traditional molecular biology and while they require

traditional care, a researcher that can reproducibly generate beautiful Northern or Southern

blots should have no difficulty generating beautiful array hybridizations.
Data management is a more recent problem for most biologists. The bulk of Part B provides a

range of techniques for data handling. This includes critical issues, from normalization within

and between arrays, to uploading your results to the public repositories for array data, and

how to integrate data from multiple sources. There are chapters in Part B for both the debutant

and the expert bioinformatician.
Provides an overview of platforms
Includes experimental design and wet bench protocols
Presents statistical and data analysis methods, array databases, data visualization and meta

analysis

Links

http://linkgrabber.blogspot.com/2006/11/dna-microarrays-part-b.html




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