DNA Repair Protocols, Eukaryotic Systems
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Description
Title: DNA Repair Protocols, Eukaryotic Systems
Description:
Pages: 641
From the Publisher
This groundbreaking manual provides step-by-step methods covering all aspects of the cellular
response to DNA damage, including nucleotide and base excision repair, DNA strand break repair,
mismatch repair and tolerance mechanisms. The techniques are based on eukaryotic model systems
in widespread use that have made, or have the potential to make, important contributions to our
understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis,
and the cell cycle. The majority of the chapters are devoted to mammalian cells, which reflects
the allocation of resources in the DNA repair field. Non-mammalian model systems are also
covered, and specifically yeasts, drosophila, Xenopus and plants, the latter of which are of
vital interest given the problem of ozone depletion and consequent loss of UV protection. The
practical methods offered in this book will encourage the transfer of methodologies between
model systems and stimulate the reader's development of new approaches in the study of DNA
damage and repair.
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