Fibrosis Research: Methods and Protocols (Methods in Molecular Medicine Volume 117)

Description

Fibrosis Research: Methods and Protocols (Methods in Molecular Medicine Volume 117)

Publisher: Humana Press
Number Of Pages: 408
Publication Date: 2005-08-08
Sales Rank: 1243557
ISBN / ASIN: 158829479X
EAN: 9781588294791
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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