In Situ Detection of DNA Damage

Description

Title: In Situ Detection of DNA Damage
Description:
Methods and Protocols (Methods in Molecular Biology, Vol 203)
(Methods in Molecular Biology)
Pages: 334

In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of

experts describe all the major in situ techniques for studying DNA damage and apoptosis, and

how approaches originally designed to label apoptotic cells can be used for DNA damage analysis

(and vice versa). The readily reproducible techniques presented here include all those designed

to detect specific single- and double-stranded DNA breaks in tissue sections while using

polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that

use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ

applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and

apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and

Protocols presents for the first time a complete set of enzymatic methods for determining DNA

breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA

damage and the labeling of apoptotic cells.

Links

http://linkgrabber.blogspot.com/2006/11/in-situ-detection-of-dna-damage.html

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