Making and Using Antibodies: A Practical Handbook

Description

Publisher: CRC
Language: English
ISBN: 0849335280
Paperback: 408 pages
Data: Dec 2006
Format: PDF
Description: Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers- ¡¤ Commercially available adjuvants designed for the production of antisera in the research setting ¡¤ Methods for the production, purification, and characterization of antibodies ¡¤ Practical guidance to researchers needing to modify antibodies ¡¤ Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry ¡¤ Methods for applying immunolabeling to the realm of electron microscopy (EM) ¡¤ New antibody sources and new ways to use antobodies Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.

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